How to write a publishable manuscript

My mentor gave our lab a presentation recently on how to write a publishable manuscript. He was elected into the National Academy of Sciences last month and has tons of publications. Therefore, I recorded some bullet points of his presentation and hope it will help when you are about to write your own manuscripts. Abstract/Summary The best of your paper Key findings and conclusions Significance of your findings Be sure that conclusion is supported by data Write after you finish your entire paper (can write first but need to revise the abstract at the end) Introduction Short overview on the topic